KMID : 0357820160400010027
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Korean Journal of Legal Medicine 2016 Volume.40 No. 1 p.27 ~ p.31
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Mixture Patterned Short Tandem Repeat Profile in a Perimortem Transfused Patient
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Seo Hee-Jin
Cho So-Hee Lee Ji-Hyun Lyoo Sung-Hee Park So-Hyung Lee Soong-Deok
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Abstract
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Recently, it has been reported that transfused patients can generate admixture-like genetic profiles. As genetic material of the donor can survive for a reasonable time after transfusion, the recipient¡¯s genomic DNA is likely to have a mixture pattern. An autopsy case of a man transfused perimortem generated a mixture patterned short tandem repeat profile. Notably, the patient was transfused mostly with nuclear-deficient cells, limiting the donor genetic material available for the recipient. As a result, mixture-like patterns were observed consistently, regardless of change in input DNA content; the sample DNA content, which was serially diluted, ranged from 1 ng to 0.0625 ng. The distributions of foreign peaks appeared to be irreproducible, showing stochastic behaviors throughout the genotyped results. This study suggests that a cautious approach is required when genotyping of a patient who has undergone recent transfusion. One must consider the possibility of obtaining a mixture patterned profile in such patients, and therefore, choose parenchymal organs or tissues for reliable results.
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KEYWORD
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Perimortem, Blood transfusion, DNA profiling, Microsatellite repeats
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